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truChIP Chromatin Shearing Reagent KIT with Formaldehyde

 
$175.00
SKU:
520154
Qty:
 
 

The truChlP Chromatin Shearing Kit is a reagents only kit optimized for the efficient and reproducible shearing of chromatin from adherent and suspension mammalian cell lines using the Covaris AFA Focused-ultrasonicators. Focused-ultrasonicators provide a non-contact, isothermal method of shearing chromatin without compromising the structural integrity of the epitopes of interest for use in ChlP-qPCR (3), ChlP-Chip (1), and ChlP-Seq (2) applications.

Kit Contents:

  • Buffer A    10X Fixing Buffer (7.5 mL)
  • Buffer B    5X Lysis Buffer (5 mL)
  • Buffer C   10X Wash Buffer (2.5 mL)
  • Buffer D3 10X SDS Shearing Buffer (Contains 1mM EDTA, 10mM Tris-HCl pH 7.6, 0.1% SDS in 1X solution) (6mL)
  • Buffer E   1X Quenching Buffer (6 mL)
  • Buffer F   100X Protease Inhibitor cocktail (0.8 mL)
  • Formaldehyde 5x1ml

Note: Processing tubes, and tube holders/racks will have to be purchased separately depending on Covaris intrument being used. Please use the list below as a guide to ordering the correct tubes/holders/racks for your system.

 

M220:

  • 520045 microTUBE AFA Fiber Pre-Slit Snap-Cap 6x16mm (25)
  • 520130 milliTUBE 1 ml AFA Fiber (100)
  • 500414 M220 Holder XTU
  • 500489 M220 Holder XTU Insert microTUBE 130 µl
  • 500422 M220 Holder XTU Insert milliTUBE 1 ml

S-Series:

  • 520045 microTUBE AFA Fiber Pre-Slit Snap-Cap 6x16mm (25)
  • 520130 milliTUBE 1 ml AFA Fiber (100)
  • 500114 Holder microTUBE
  • 500371 Holder milliTUBE 1 ml

E & L-Series:

  • 520045 microTUBE AFA Fiber Pre-Slit Snap-Cap 6x16mm (25)
  • 520130 milliTUBE 1 ml AFA Fiber (100)
  • 520053 8 microTUBE Strip (12
  • 520078 96 microTUBE Plate
  • 500329 Rack-XT 96 microTUBE Plate
  • 500282 Rack 96 Place microTUBE
  • 500191 Rack 12 place 8 microTUBE Strip
  • 500368 Rack 24 Place milliTUBE 1 ml

 

Optimized reagents and protocol for chromatin shearing with AFA. Kit includes formaldehyde. Requires two day shipping and an added shipping fee for hazardous materials. This part replaces Covaris Covaris 520127.

 

Case quantity available 520155.

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Orders will be processed within 24 to 48 hours of placing the order.